“Single-cell and spatial dissection of plant-microbe interactions”
Dr. Tatsuya Nobori
HFSP Fellow (Joseph Ecker Lab)
Salk Institute, San Diego, California, USA
Date: 30 November 2022
Time: 16:00 – 17:00
Location: online : https://universiteitleiden.zoom.us/j/61775029733?pwd=ZHpZNUY3eFJsbUIvSGdTdEZZdENJUT09#success
Cells comprising plant tissues process numerous developmental and environmental cues
with cell-specific gene regulatory principles to manifest highly heterogeneous
responses. Retrieving the complex gene regulation of individual cells in the native
three-dimensional (3D) tissue context is the cornerstone of a more complete
understanding of tissue functions.
In this seminar, I will introduce (1) the application of a single-cell multiomics
technology to the model plant Arabidopsis thaliana and (2) the development of a new
method for multiplexed single-cell 3D spatial gene expression analysis in wholemount
plant tissue. I will discuss how integrating these approaches can uncover plantmicrobe
interactions at high resolution.
Tatsuya Nobori is a Human Frontier Science Program (HFSP) postdoctoral fellow in the lab
of Prof. Joe Ecker at the Salk Institute in San Diego, California. He completed his Ph.D. in 2019 in the
group of Dr. Kenichi Tsuda at the Max Planck Institute for Plant Breeding Research in Cologne,
Germany, where he established methods to analyze bacterial transcriptomes and proteomes in planta.
His studies revealed how plants influence the responses of pathogens and microbiota. Tatsuya’s
current research goal is to understand plant-microbe interactions at the single-cell resolution by
incorporating and developing new sequencing and imaging technologies.
You can follow him on Twitter, @nobolly.
Wednesday, November 30, 2022