GLS Seminar, Dr. Nadine Paris

Host: Francesca Quattrocchio 
Speaker: Dr. Nadine Paris, Biochemistry and Plant Molecular Physiology, Equipe KaliPHruit, CNRS
Title: “Use of ratiometric fluorescent pH sensors in plant cells – potential and limitation”

Proton homeostasis is known for a long time as essential for plant cell functions especially in the acidic growth theory that was first proposed almost 50 years ago. More recently, the development of powerful fluorescent microscopes together with an exponentially growing set of pH sensors has opened a new era of real time imaging to study proton distribution at high time and scale resolution. In my talk, I will focus on ratiometric fluorescent sensors for pH that are genetically encoded. I will describe the potential and limitation of such sensors through the examples of protein trafficking in plant cells, ionic exchange between plant root and its environments. Finally I will detail our current and further research project where pH sensors are used to study cell elongation processes.

Literature:
Martinière A, Gibrat R, Sentenac H, Dumont X, Gaillard I and Paris N. Uncovering pH at both sides of the root plasma membrane interface using noninvasive imaging. (2018) Proc. Natl. Acad. Sci. USA. 115, 6488-6493.

Martinière A., Bassil E, Jublanc E, Alcon C, Reguera M, Sentenac H, Blumwald E and Paris N. In vivo intracellular pH measurements in tobacco and Arabidopsis reveal an unexpected pH gradient in the endomembrane system (2013). Plant Cell 25, 4028-4043.

Thursday, January 31, 2019 through February 1
11:00 - 12:00
Science Park 904
Science Park 904, Amsterdam