Postponed!!! Advanced Course ‘Microscopy and Spectroscopy in Food and Plant Sciences’
Dates: 5-7 July 2022
Venue: Wageningen Campus, Wageningen, The Netherlands
Target group: PhD candidates and postdocs, maximum of 24 participants
Fee: €180 for VLAG or EPS PhD candidates with an approved TSP or postdocs registered at VLAG or EPS; €360 for all other PhD candidates and postdocs; €720 for all others.
Registration: Closed.
Language: English
Study credits: 0.9 ECTS
Lecturers: Jan Willem Borst (WUR-Biochemistry), Tijs Ketelaar (WUR-Cell Biology), Norbert de Ruijter (WUR-Cell Biology), Peter van Gisbergen (WUR-Cell Biology), Arjen Bader (WUR-Biophysics) & Johannes Hohlbein (WUR-Biophysics)
Organisation: The Graduate Schools VLAG and EPS
Introduction:
The MicroSpectroscopy Research Facility and the Wageningen Light Microscopy Centre of Wageningen University will organize a VLAG & EPS funded advanced practical course for postdocs and PhD candidates. The course will cover the theory behind several advanced microscopic and micro-spectroscopic techniques that have a wide range of applications in both the Food and Plant Sciences.
The following topics will be treated during the lectures with examples of application in Food and Plant sciences: microscopy, fluorescence spectroscopy, confocal microscopy, Förster Resonance Energy Transfer (FRET) imaging, Fluorescence Lifetime Imaging Microscopy (FLIM), image analysis, 3D Imaging, time- and spectrally-resolved fluorescence spectroscopy, total internal reflection fluorescence (TIRF), super-resolution imaging and multi-photon imaging.
The hands-on training during this course will be in the form of group assignments. For additional individual training after this course, we would like to refer you to the MicroSpectroscopy Research Facility and the Wageningen Light Microscopy Centre.
Provisional program
Tuesday 18 January
09:00 Intro and “Light microscopy; creating contrast” (Ketelaar)
09:45 break
10:00 lecture “Fluorescence microscopy and optical sectioning” (Ketelaar)
10:45 break
11:00 Start computer practical “digital image analysis” (Bader/deRuijter/Borst)
13:00 lunch
14:00 tour in 4 groups showing basic microscopy, spectroscopy techniques, sample preparation, confocal microscopy (Bader/deRuijter/Borst/VanGisbergen)
18:00 Dinner
Wednesday 19 January
09:00 lecture ““Fluorescent probes” (Borst/Schmid)
10:00 break
10:15 lecture “Förster resonance energy transfer” (Borst)
10:45 break
11:00 lecture “Single Molecule Techniques” (Hohlbein)
12:00 lunch
13:30 Practical 1,2,3 and 4 (depending on group assignment)
15:30 Change practical: Practical 1,2,3 and 4 (depending on group assignment)
17:30 End of day 2
Thursday 20 January
09:00 lecture “Advanced (functional) imaging applications” (Borst)
09:45 break
10:00 Practical 1,2,3 and 4 (depending on group assignment)
12:00 lunch
13:00 Practical 1,2,3 and 4 (depending on group assignment)
15:00 Data analysis
17:30 Evaluation and drinks
Practical 1: Spinning disk imaging
Practical 2: Confocal (3D) imaging
Practical 3: (FRET)-FLIM
Practical 4: Multi-photon imaging
Tuesday, July 5, 2022 through July 7
all day long
Wageningen Campus
Bronland 1, Wageningen