Advanced Course ‘Microscopy and Spectroscopy in Food and Plant Sciences’
Dates: 18 – 20 January 2022
Venue: Wageningen Campus, Wageningen, The Netherlands
Target group: PhD candidates and postdocs, maximum of 24 participants
Fee: €180 for VLAG or EPS PhD candidates with an approved TSP or postdocs registered at VLAG or EPS; €360 for all other PhD candidates and postdocs; €720 for all others.
Registration: here (Deadline 1 January 2022).During the registration, you will be ask to fill in your motivation. If there are more registrations than places available, the motivation letter will be used for the selection procedure.
Study credits: 0.9 ECTS
Lecturers: Jan Willem Borst (WUR-Biochemistry), Tijs Ketelaar (WUR-Cell Biology), Norbert de Ruijter (WUR-Cell Biology), Peter van Gisbergen (WUR-Cell Biology), Arjen Bader (WUR-Biophysics) & Johannes Hohlbein (WUR-Biophysics)
Organisation: The Graduate Schools VLAG and EPS
The MicroSpectroscopy Research Facility and the Wageningen Light Microscopy Centre of Wageningen University will organize a VLAG & EPS funded advanced practical course for postdocs and PhD candidates. The course will cover the theory behind several advanced microscopic and micro-spectroscopic techniques that have a wide range of applications in both the Food and Plant Sciences.
The following topics will be treated during the lectures with examples of application in Food and Plant sciences: microscopy, fluorescence spectroscopy, confocal microscopy, Förster Resonance Energy Transfer (FRET) imaging, Fluorescence Lifetime Imaging Microscopy (FLIM), image analysis, 3D Imaging, time- and spectrally-resolved fluorescence spectroscopy, total internal reflection fluorescence (TIRF), super-resolution imaging and multi-photon imaging.
The hands-on training during this course will be in the form of group assignments. For additional individual training after this course, we would like to refer you to the MicroSpectroscopy Research Facility and the Wageningen Light Microscopy Centre.
Tuesday 18 January
09:00 Intro and “Light microscopy; creating contrast” (Ketelaar)
10:00 lecture “Fluorescence microscopy and optical sectioning” (Ketelaar)
11:00 Start computer practical “digital image analysis” (Bader/deRuijter/Borst)
14:00 tour in 4 groups showing basic microscopy, spectroscopy techniques, sample preparation, confocal microscopy (Bader/deRuijter/Borst/VanGisbergen)
Wednesday 19 January
09:00 lecture ““Fluorescent probes” (Borst/Schmid)
10:15 lecture “Förster resonance energy transfer” (Borst)
11:00 lecture “Single Molecule Techniques” (Hohlbein)
13:30 Practical 1,2,3 and 4 (depending on group assignment)
15:30 Change practical: Practical 1,2,3 and 4 (depending on group assignment)
17:30 End of day 2
Thursday 20 January
09:00 lecture “Advanced (functional) imaging applications” (Borst)
10:00 Practical 1,2,3 and 4 (depending on group assignment)
13:00 Practical 1,2,3 and 4 (depending on group assignment)
15:00 Data analysis
17:30 Evaluation and drinks
Practical 1: Spinning disk imaging
Practical 2: Confocal (3D) imaging
Practical 3: (FRET)-FLIM
Practical 4: Multi-photon imaging
Tuesday, January 18, 2022 through January 20
all day long
Bronland 1, Wageningen