Advanced Course – Transcription Factors and Transcriptional Regulation
Introduction
Gene regulation is an essential process in all organisms. Transcription of genes is tightly controlled in a temporal and spatial manner and responds to internal and external signals, such as developmental cues, temperature and pathogen attack. Eukaryotes have evolved numerous mechanisms to activate or suppress gene expression, among others by the action of specific transcription factors (TF), chromatin remodelling, histone modifications, DNA methylation, histone variants, alternative splicing, and microRNAs. In this EPS course, an attractive program is provided in which experts in the field will highlight and discuss different aspects of gene regulation.
Content
In addition to lectures by experts, there will be ample opportunity for discussions during poster viewing sessions and in a literature discussion session. Furthermore, in a short computer workshop you will learn how to analyse and find TF binding sites using ChIP-seq and other NGS data. Topics that will be addressed are as follows:
Day 1: Introduction on transcriptional regulation, related technologies, building Gene Regulatory Networks and usage of websites for the analysis of TFs and binding sites.
Day 2: Epigenetic regulation (DNA methylation, chromatin conformation and modifications).
Day 3: A number of interesting lectures about plant TFs and transcriptional regulation. A more detailed schedule will follow later.
The course is especially attractive for PhD candidates, but the program will also be of interest for post-docs and senior scientists who are working in the field of gene regulation or are using related technologies.
Provisional Programme:
Monday, December 10 Technologies and Approaches, General Principles
Venue: Orion room C3034
10.00-10.05 Introduction and outline of the workshop
10.05-10.50 Gerco Angenent (WUR): ‘Introduction about transcription factors and their features’
10.55-11.20 Break, poster set-up (C3043)
11.20-11.55 Gerco Angenent (WUR): ‘TF related technologies’
12.00-12.30 Poster pitches, 2 min each
12.30-13.30 Lunch and poster session (C3043)
13.30-14.15 Maike Stam (UvA): ‘Introduction in epigenetic mechanisms’
14.20-15.05 Basten Snoek (UU): ‘Experimental and computational mapping of gene regulatory networks’
15.10-15.40 Break (C3043)
15.40-17.00 Aalt-Jan van Dijk (WUR): ‘Introduction bioinformatics practical’ (PC-room PC4044/PC4045)
Tuesday, December 11 Chromatin and Transcription
Venue: Orion room C3034
09.30-10.30 Aalt-Jan van Dijk (WUR)( PC-room PC4044/PC4045): ‘Practical in database analysis, bioinformatics and promoter and binding site analysis’
10.30-10.50 Break (C3043)
10.55-11.35 Jan Kooter (VU): ‘An introduction in DNA methylation’
11.40-12.10 Poster pitches
12.15-13.15 Lunch and poster session (C3043)
13.20-14.05 Lionel Navarro (CNRS, Paris, France): ‘Transcriptional control of immune- responsive genes by DNA methylation and demethylation and its relevance in antibacterial defense’
14.10-14.40 Maike Stam (UvA): ‘Enhancers and chromatin conformation’
14.45-15.10 Break (C3043)
15.15-16:00 Isabel Bäurle (University Potsdam, Germany): “How do plants remember a stressful day – a role for epigenetics?!”
16.05-16.50 Daniel Zilberman (JIC, Norwich, UK): DNA methylation
Wednesday, December 12 Specific talks about Transcriptionnal Regulation
Venue: Orion room C3034
09.30-10.30 Literature discussion in groups.
10.30-10.50 Break (C3043)
10.55-11.25 Corné Pieterse (UU): ‘Hug the hubs: gene regulatory networks in plant immunity’
11.30-12.00 Viola Willemsen (WUR): ‘PLTs, TCPs, and birds’
12.05-12.50 Lunch (C3043)
12.55-13.45 Bas van Steensel
13.50-14.25 Koen Verhoeven (NIOO): ‘Eco-epigenetics’
14.30-15.00 Harrold van den Burg (UvA): ‘SUMO modification and gene expression’
15.05-15.30 Break (C3043)
15.35-16.05 Richard Immink (WUR): ‘About chromatin, splicing and Long non-coding RNAs’
16.10-16.40 Dolf Weijers (WUR): ‘Mechanisms of transcription factor activity and specificity in the auxin pathway’
16.50: Closing & Drinks
Keywords: Transcription factors and regulatory sequences, Gene regulation, ChIP-seq, microRNAs, Chromatin remodelling, Epigenetic regulation (a.o. DNA methylation, histone modifications)
Language: English
Group size: maximum of 40 participants
Credits: 1.0 ECTS
Fee: € 150 for EPS PhD students with approved TSP or postdocs registered with EPS;
€ 300 for other PhD students, postdocs and academic staff;
€ 450 for participants from the private sector.
Registration: closed! (please be aware that you will need to bring a poster of your work to the course)
Organizers: Gerco Angenent (Wageningen University) and Maike Stam (University of Amsterdam)
Contact: Susan Urbanus
Monday, December 10, 2018 through December 12
10:00 - 17:30 daily
WUR – Orion
Bronland 1, Wageningen