Basic Course ‘Electron Microscopy from A to W’

Registration is now closed

Introduction
In recent years, Electron Microscopy (EM) has rapidly developed as a powerful research tool for high-resolution imaging, leading to high-ranking papers, a Nobel price and recently in The Netherlands to a big NWO award. New, more powerful electron microscopes and the possibility to work at very low temperatures (cryo-EM), have made it possible to achieve atomic resolutions. In addition, combined light and electron microscopy (CLEM; correlative microscopy) now allows precise detection of fluorescently labelled molecules in high resolution images of cells and tissues.
During this course, you will be introduced to all these novel developments. You will learn to use both transmission (TEM) as well as scanning electron microscopes (SEM) with different detectors. You will perform classical techniques for specimen preparation, like resin embedding of cells and tissues and negative staining of small structures like virus particles, as well as cryo-techniques for both TEM and SEM. The course will also include tips and tricks to perform CLEM.

Aim and scope
After this course, you will have insight into the possibilities of electron microscopy as a research tool and are able to decide whether you should use SEM, TEM or CLEM for your analysis. You will be able to operate an EM at basic level, make images and perform basic techniques of sample preparation. To benefit optimally from this course you will need some understanding of microscopes in general, but it is not necessary to have experience with EM.

Format and program
4 Days practical training at WUR and AMC locations and a final day with presentations of participants, guest lectures on advanced microscopy and concluding reception at AMC. Please find more detailed information about the program in this document and also on www.cellularimaging.nl.

Participants: 16 PhD students from AMC-UvA, EPS or WUR.
Language: The course is given in English.
Study load: 44 hours, 1.6 ECTS points.

Course organizers
Jan van Lent (WUR), Marcel Giesbers (WUR), Nicole van der Wel (AMC) and Henk van Veen (AMC).
For more information about the course itself, please contact Nicole van der Wel or Jan van Lent.

Registration and costs for EPS or WUR PhD students
Registration is now closed. Participation cannot be guaranteed beforehand because of the limited group size. The selection of participants will among others be based on the short motivation of how you intend to apply the knowledge from this EM course in your own research. Participation of selected registrants will be confirmed before October 1st 2018. The selected participants will receive an invoice. Payment will be only possible by invoice (no credit cards and no cash payment on site).
Participation fee is €250 for PhD students of the Graduate Schools EPS / WIMEK-SENSE / PE&RC / WIAS / VLAG; €500 for other PhD students from WUR. Lunch and concluding reception are included in the course fee.

Registration for AMC-UvA PhD students
Please contact Claudia Rhebergen, e-mail: c.rhebergen@amc.uva.nl, tel. +31 (0)20 566 4950.

Monday, November 12, 2018 through November 16
Program will follow
Amsterdam UMC, locatie AMC
Meibergdreef 9, Amsterdam