Advanced PhD Course Proteomics (hands-on)

20-23 October 2008, Wageningen University, Wageningen, The Netherlands

Organized by The Graduate Schools VLAG and EPS, and Department of Biochemistry, Wageningen University

Course description:
The course is a 4 days hands-on course with a maximum of 16 participants (PhD's and postdocs are the target group). In the morning sessions lectures on mass spectrometry and proteomics applications and in the afternoon sessions practical work are scheduled. Participants should bring their own sample and one slice of a gel (1D gel is preferred, but 2D gels are also possible, advise on the selection criteria will be provided) will be measured using splittless nano-HPLC Orbitrap FTMS.
In view of the limited number of participants (max 16), PhD students and postdocs applying will be selected. Each participant should therefore indicate in a half page A4 the background of her/his project.

• Programme topics:
  
• • introduction to mass spectrometry
• • spectral interpretation
• • 1D and 2D gel electrophoresis
• • digestion of samples
• • preparation of samples for FTMS measurements (spot picking, in gel digestion)
• • introduction to databases
• • database searching
• • evaluation using Bioworks,
• • Mascot and OMSSA
• • LC-ESI-MS
• • Quantitative techniques
• • exercise in data-evaluation of peptide mass
• • fingerprinting data and of LC-MS data sets
• • measurements of intact proteins and protein-ligand interaction using MS.
• • Search for biomarkers using FT-MS
• • data evaluation of own FT-MS data
• • biostatistical tools for the exploration and interpretation of proteomics data
• • nutrition-related changes in tissue and plasma proteomes-insights from 2D gel electrophoresis studies
• • Presentation by each participant of the results

Course coordinators:
• Jacques Vervoort, Sjef Boeren, Rumyana Karlova - Laboratory of Biochemistry - WU
• Baukje de Roos - Rowett Research Institute, Aberdeen, Scotland
• Ingeborg van Leeuwen-Bol - Graduate School VLAG

Lecturers:
• Graham Horgan, Claus Mayer - Rowett Research Institute, Bioss, Aberdeen, Scotland
• Robert van den Heuvel - Organon-Schering-Plough
• Peter Burgers - Erasmus University, Rotterdam

Language:
The course will be conducted in English.

Location & Accommodation:
Lectures and practicals will be given at: Wageningen University, Transistorium building, no. 312 (location ‘The Dreijen’), Dreijenlaan 3, 6703 HA Wageningen, The Netherlands

A number of hotel rooms have been blocked for course participants at the WICC, but only until September 29th, 2008. Accommodation costs are about € 75 (single room) per night incl. breakfast, excl Tax. Participants have to book their own hotel room. Hotel reservation is handled by WICC, Phone: +31-317-490133, http://www.wicc.nl/. Mention booking code: "Pro'08".

Contact information:
More information about the course contents can be obtained from:
Dr. Jacques Vervoort, This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

For organizational matters please contact:
Mrs. I. van Leeuwen-Bol, This e-mail address is being protected from spam bots, you need JavaScript enabled to view it
Phone: +31-317-486108

Registration & Course Fee:
The course fee includes coffee/tea during the breaks, lunches, course material and one course dinner.
University staff/ Non -Profit: € 700
PhD students: € 400
VLAG/EPS PhD students: € 200

Cancellation may be made free of charge until August 22nd, 2008. After this date the charge will be 25% of the fee paid or due. Substitutions may be made at any time until the start of the course.
Theregistration form, including background information (half page A4 with background on her/his project) must be returned before August 22nd, 2008. Please, use our automatic registration form, the background information may be send to This e-mail address is being protected from spam bots, you need JavaScript enabled to view it (mention 'Proteomics' in the subject line of the message). You will be notified on acceptance after August 28th, and instructions for payment, a letter of acceptance and further course details will be sent to you by e-mail.